polymicrobial cultures  (BioFire Defense)

Journal: ACS infectious diseases

Article Title: Self-assembly of antimicrobial peptoids impacts their biological effects on ESKAPE bacterial pathogens

doi: 10.1021/acsinfecdis.1c00536

Figure Lengend Snippet: Effect of peptoids under host-mimicking conditions on mono- and polymicrobial biofilm eradication. (A) P. aeruginosa LESB58 (5 × 105 CFU/ml) and (B) S. aureus USA300 LAC (2.5 × 107 CFU/ml) mono- and (C) polymicrobial biofilms were grown for 20-24 h in DMEM-FBS-G prior to treatment with peptoids. Biofilms were stained with crystal violet (0.1%) after an additional 24 h. Values were normalised to the biofilm growth control, which is indicated by the dotted line. Data from three independent experiments (n = 3) are presented as the mean ± SEM.

Article Snippet: For polymicrobial cultures selective agar plates were used: Pseudomonas cetrimide agar (Oxoid) to select for P. aeruginosa and 7.5% NaCl plates to select for S. aureus .

Techniques: Staining, Control

Journal: ACS infectious diseases

Article Title: Self-assembly of antimicrobial peptoids impacts their biological effects on ESKAPE bacterial pathogens

doi: 10.1021/acsinfecdis.1c00536

Figure Lengend Snippet: Effect of peptoids on mono- and polymicrobial biofilms. Peptoids (31.25 μg/ml) were used to treat biofilms comprising (A,B) P. aeruginosa LESB58 (5 × 105 CFU/ml), (C,D) S. aureus USA300 LAC (2.5 × 107 CFU/ml) or (E,F) both species. Biofilms were grown for 20-24 h in DMEM-FBS-G prior to treatment and re-incubated for another 20-24 h. (A,C,E) Biofilm was quantified by CV staining (%) and (B,D,F) and bacterial recovery from biofilms (CFU/ml) determined on selective agar plates. The dotted line indicates the limit of detection (LOD) at 102 CFU. Data from three independent experiments each (n = 3) are shown as (A,C) mean ± SEM or geometric mean ± geometric SD. * P < 0.05, ** P < 0.01 according to Kruskal-Wallis test with Dunn’s correction.

Article Snippet: For polymicrobial cultures selective agar plates were used: Pseudomonas cetrimide agar (Oxoid) to select for P. aeruginosa and 7.5% NaCl plates to select for S. aureus .

Techniques: Incubation, Staining